Studies of the Mechanism of Anthranilate Synthase
نویسندگان
چکیده
منابع مشابه
Studies of the mechanism of anthranilate synthase reaction.
The enzyme anthranilate synthase catalyzes the formation of anthranilate form either chorismate and glutamine or chorismate and ammonia. In the aromatization of chorismate, a hydroxyl group and an enolpyruvyl group must be eliminated. Elimination of the enolpyruvyl group of chorismate is accompanied by protonation to form pyruvate. The source of this proton was investigated by performing the en...
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چکیده ندارد.
15 صفحه اولStudies of the mechanism of anthranilate synthase. Evidence for an acyl-enzyme.
The mechanism of anthranilate synthase reaction was studied using hydroxylamine as an inhibitor. Changes in the NH,OH concentration result in a concomitant alteration in the rates of synthesis of y-glutamylhydroxamate and anthranilate. However, the sum of the two products appears to be constant. Moreover, the degree of inhibition elicited by NHsOH at different concentrations is similar for the ...
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The crystal structure of anthranilate synthase (AS) from Serratia marcescens, a mesophilic bacterium, has been solved in the presence of its substrates, chorismate and glutamine, and one product, glutamate, at 1.95 Å, and with its bound feedback inhibitor, tryptophan, at 2.4 Å. In comparison with the AS structure from the hyperthermophile Sulfolobus solfataricus, the S. marcescens structure sho...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62285-9